KMID : 1098420180260060471
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Korean Journal of Medicinal Crop Science 2018 Volume.26 No. 6 p.471 ~ p.481
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Selection of Short Stem Astragalus membranaceus Lines by Assessing Agronomic Characteristics and Biological Activity
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Lee Sang-Hoon
Koo Sung-Cheol Han Jong-Won Lee Woo-Moon Hur Mok
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Abstract
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Background: Astragalus membranaceus belonging to the Leguminosae family is often utilized as a traditional medicine. The aim of this study was to elucidate the basic breeding information required to develop short stem A. membranaceus cultivars.
Methods and Results: Roots of A. membranaceus advanced yield trial (AYT) lines were harvested in late October 2017. Root yield of six AYT lines were increased in a range of 8.9 - 74.8% compared with ¡®Aseong¡¯ as control (check variety). The height of seven AYT lines were shorter than that of ¡®Aseong¡¯. In addition, stem diameter of nine AYT lines was thicker than that of ¡®Aseong¡¯. Consequently, 1502-56, 1503-90, and 1510-80 were selected as elite lines for the development of short stem cultivars. HPLC analysis was perfromed to identify lines with high level active components such as calycosin-7-O-¥â-D-glucoside and calycosin. The levels of both active components were higher in 1502-56, and 1503-90, but not in 1510-80 compared to ¡®Aseong¡¯. In addition, 2,2-Diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity was higher in the 1502-56 compared to ¡®Aseong¡¯. Considering these results, two AYT lines, 1502-56 and 1503-90 were selected as short stem lines with high calycosin-7-O-¥â-D-glucoside and calycosin content.
Conclusions: Taken together, Two short stem lines were identified in this study. In our future study, regional yield trial (RYT) will be conducted using these selected lines to develop new cultivars.
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KEYWORD
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Astragalus membranaceus, Calycosin-7-O-¥â-D-glucoside, Calycosin, High Yield, Short Stem
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